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  • In vitro selection and plant regeneration of copper

    Copper tolerance of Nicotiana tabacum L. var. Xanthi in vitro was achieved through plant regeneration from leaf explants on Murashige and Skoog's (MS) medium supplemented with 0.5 mg/l BA, 0.1–0.25 mg/l IAA and 60 μ m Cu. Tolerant organogenic calli showed more vigorous growth in medium containing 60 μ m Cu than the non‐tolerant calli. . Standard growth parameters such as fresh and dry

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  • The role of nitrilotriacetate in copper uptake by tobacco

    In growth chamber experiments we studied the effect of nitrilotriacetate (NTA) on Cu uptake by tobacco (Nicotiana tabacum L.). Plants were exposed for 6 d to 126 microM Cu and 500 microM NTA in nutrient solutions without and with 10 g L(-1) montmorillonite. Approximately seven times less Cu was diss The role of nitrilotriacetate in copper uptake by tobacco J Environ Qual. Sep-Oct 2003;32(5

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  • Transgenic Nicotiana tabacum plants expressing a fungal

    Copper is an essential element, which is often deficient in human diet. With the objective of developing plants with improved copper acquisition, a high-affinity copper transporter gene (tcu-1) was cloned from fungus Neurospora crassa and introduced into a model plant (Nicotiana tabacum). Integration of the transgene was confirmed by Southern blot hybridization. Transgenic tobacco plants (T(0

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  • Nicotiana tabacum an overview ScienceDirect Topics

    Nicotiana tabacum cells, or cultivated tobacco, are the most commonly cultured plant suspension cells in stirred tank and airlift bioreactors because genetic transformation and propagation of these cells are simple and the methods are well established. 18 Scientists have focused on suspension cultures of plant cells because they are the most amenable to large-scale production capabilities and are

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  • Nicotiana tabacum, Tobacco seeds

    Nicotiana tabacum belongs to the family of the Solanaceae, the nightshades. It is native to America but today it is grown all over the world. Jean Nicot brought N. tabacum 1560 to France. In honor to that the plant was named Nicotina. N. tabacum is also known as cultivated Tobacco. N. tabacum is probably a natural hybrid between Nicotina sylvestris and Nicotina tomentosiformis. N. tabacum is

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  • Characteristics of heavy metal uptake by plant species

    01.05.2000· In contrast, growth of Nicotiana tabacum hairy roots was severely retarded at (Robinson et al., 1997). In order to assess the feasibility of phytoremediation and phytomining, it is necessary to quantify the relationships between environmental conditions, including metal concentration, and growth and metal uptake by plant tissues. As roots are the plant organs that come into direct contact

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  • Nicotiana tabacum (tobacco) CABI.org

    The leaves of Nicotiana plants produce leaf wax toxins on their surface in high concentrations to inhibit blue mould and fungal infections, and N. tabacum has also been studied for its production of phytoalexins as a major defense mechanism in response to microbial infections (Harbone, 1986).

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  • Nicotiana tabacum Wikipedia

    Nicotiana tabacum, or cultivated tobacco, is an annually-grown herbaceous plant. It is found in cultivation, where it is the most commonly grown of all plants in the genus Nicotiana, and its leaves are commercially grown in many countries to be processed into tobacco. It grows to heights between 1 and 2 meters. Research is ongoing into its ancestry among wild Nicotiana species, but it is believed to be a

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  • Nicotiana tabacum — Wikipédia

    Nicotiana tabacum, le tabac ou tabac cultivé, est une espèce de plante dicotylédone annuelle de la famille des Solanacées, originaire d'Amérique centrale, largement cultivée pour ses feuilles séchées riches en nicotine qui servent à la préparation du tabac manufacturé. Il est parfois appelé grand tabac et plus rarement herbe à Nicot

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  • The role of nitrilotriacetate in copper uptake by tobacco

    In growth chamber experiments we studied the effect of nitrilotriacetate (NTA) on Cu uptake by tobacco (Nicotiana tabacum L.). Plants were exposed for 6 d to 126 microM Cu and 500 microM NTA in nutrient solutions without and with 10 g L(-1) montmorillonite. Approximately seven times less Cu was dissolved in the montmorillonite solutions than in the nutrient solutions alone. In the absence of NTA,

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  • Copper-mediated oxidative burst in Nicotiana tabacum L.

    In cell suspension cultures of Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) a rapid and concentration-dependent accumulation of H2O2 is induced by excess concentrations of copper (up to 100 μM). This specific and early response towards copper stress was shown to be extracellular. Addition of 300 U of catalase per ml decreased the level of H2O2.

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  • Transgenic Nicotiana tabacum plants expressing a fungal

    With the objective of developing plants with improved copper acquisition, a high-affinity copper transporter gene (tcu-1) was cloned from fungus Neurospora crassa and introduced into a model plant (Nicotiana tabacum). Integration of the transgene was confirmed by Southern blot hybridization. Transgenic tobacco plants (T(0) and T(1)) expressing tcu-1, when grown in hydroponic medium

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  • Response of shape in vitro cultures of shape Nicotiana

    In Nicotiana tabacum L. var. BEL W3 copper (Cu) at concentrations higher than 50 μM significantly inhibited callus growth and shoot regeneration. After 5–6 months of culture only a few morphogenic callus lines survived in the presence of 100 μM Cu. These calluses showed the capacity to grow and regenerate shoots through successive subcultures on medium containing 100 μM Cu.

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  • (PDF) Copper-mediated oxidative burst in Nicotiana

    Summary. In cell suspension cultures of Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) a rapid and concentration-dependent accumulation of H2O2 is induced by excess concentrations of copper (up to 100 μM). This specific and early response towards

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  • In vitro selection and plant regeneration of copper

    Copper tolerance of Nicotiana tabacum L. var. Xanthi in vitro was achieved through plant regeneration from leaf explants on Murashige and Skoog's (MS) medium supplemented with 0.5 mg/l BA, 0.1–0.25 mg/l IAA and 60 μ m Cu. Tolerant organogenic calli showed more vigorous growth in medium containing 60 μ m Cu than the non‐tolerant calli. Standard growth parameters such as fresh and dry weight of

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  • Effect of bacterial inoculants on phytomining of metals

    01.03.2018· The plant species used in the experiment were Nicotiana tabacum and Salix smithiana. Seeds of N. tabacum were obtained from Phytotech Foundation, Switzerland. Herzig et al. (2014a) used conventional selection and in vitro breeding methods to select metal-tolerant tobacco variants with enhanced biomass and metal-uptake.

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  • Nicotiana tabacum L MAFIADOC.COM

    The aim of this study is to analyze intoxicated tobacco material (Nicotiana tabacum L.) with heavy metals (copper, cadmium, lead) and overdoses of fungicide (Ridomil gold) for enzymatic (catalase activity) and biopigment activity (chloroplast pigments) and to make morpho-anatomic examinations (lateral cut of stem and leaf). The plants were from the crop 2008 of two oriental (half oriental) types: PRILEP (P

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  • Hairy Roots In Phytoremediation And Phytomining

    Cadmium accumulation has been investigated using several species, including Nicotiana tabacum, Beta vulgaris and Calystegia sepium (71), Solanum nigrum (72), Rubia tinctorum (73), and Daucus carota (74). In these studies, aspects of metal tolerance of the hairy roots, such as induction of phyto-chelatins (73,74), and stress responses such as ethylene production and lipid peroxidation (74) were

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  • Phytomining of valuable metals from waste incineration

    Phytomining offers an environmentally sound and cheap technology to recover such metals from secondary sources. Thus, the aim of our research work is to investigate the potential of phytomining from waste incineration slags by growing metal hyperaccumulating plants on this substrates and use the metal enriched biomass as a bio-ore. As a first stage, material from Vienna's waste incineration

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